Author/Authors :
Farrokhi, Majid Reza Shiraz University of Medical Sciences, Shiraz , Emamghoreishi, Masoumeh Department of Pharmacology - School of Medicine - Shiraz University of Medical Sciences, Shiraz , Amiri, Atena Shiraz University of Medical Sciences, Shiraz , Keshavarz, Mojtaba Shiraz University of Medical Sciences, Shiraz
Abstract :
Introduction: The reduction of glycogen synthase kinase-3β protein level may
correlate to the neuroprotective effects of antioxidant agents like caffeine. Therefore,
we aimed to evaluate the impact of GSK-3β protein on neuroprotective effects of
caffeine in the SHSY5Y cells exposed to beta-amyloid.
Methods: We incubated SHSY5Ycells with beta-amyloid 25–35 and caffeine (0.6 and
1mM) for 24h. Cell viability was determined using MTT test. We used the western
blotting technique to measure the glycogen synthase kinase-3β and phosphorylated
glycogen synthase kinase-3β protein levels.
Results: Caffeine (0.6 and 1mM) diminished beta-amyloid neurotoxicity and
attenuated the beta-amyloid effects on the glycogen synthase kinase-3β protein level
in a neuronal culture.
Conclusion: Caffeine neuroprotective effects against beta-amyloid may correlate to
glycogen synthase kinase-3β protein.
Keywords :
Caffeine , Amyloid-beta peptide , Glycogen synthase kinase , Neuroprotection
