Author/Authors :
Saffari, Mojtaba Department of Medical Genetics - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran - Cancer Biology Research Center - Cancer Institute of Iran - Tehran University of Medical Sciences , Ghaderian, Mohammad Hossein Chronic Kidney Disease Research Center - Shahid Beheshti University of Medical Sciences, Tehran , Omrani, Mir Davood Urogenital Stem Cell Research Center - Shahid Beheshti University of Medical Sciences, Tehran , Afsharpad, Mandana Cancer Control Research Center - Cancer Control Fundation - Iran University of Medical Sciences, Tehran , Shankaie, Kimia Department of Biology - Science and research branch - Islamic Azad University, Tehran , Samadaian, Niusha Department of Medical Genetics - School of Medicine - Tehran University of Medical Sciences
Abstract :
Purpose: This study aims to investigate the expression level of mir-let7b-3p and mir-548, which are involved
in PTEN expression in tissue samples of prostate cancer patients versus benign prostate hyperplasia (BPH) and
normal adjacent tissue.
Materials and Methods: Prostate cancer tissues were obtained from patients after receiving informed consent.
Total RNA extraction and cDNA synthesis were performed for determining gene expression.
Results: Ten patients were determined to have high Gleason scores (> 7), 36 and seven samples had intermediate
Gleason scores (7≥) and BPH, respectively, and 40 samples were derived from normal adjacent tissue. Downregulation
of mir-let7b and upregulation of mir-548 expression significantly correlated with high-risk Gleason scores.
Conclusion: The present study showed that miR-let7b and/or mir-548 can be considered as potential targets in
prostate cancer therapy.
Keywords :
Prostate cancer , mir-let7b , mir-548 , PTEN
