Author/Authors :
Tanhaei, Somayeh Department of Reproductive Biotechnology - Reproductive Biomedicine Research Center - Royan Institute for Biotechnology - ACECR, Isfahan - Department of Cellular Biotechnology - Cell Science Research Center - Royan Institute for Biotechnology - ACECR, Isfahan , Abdali-Mashhadi, Solmaz Department of Reproductive Biotechnology - Reproductive Biomedicine Research Center - Royan Institute for Biotechnology - ACECR, Isfahan , Tavalaee, Marziyeh Department of Reproductive Biotechnology - Reproductive Biomedicine Research Center - Royan Institute for Biotechnology - ACECR, Isfahan , Javadian-Elyaderani, Soudabeh Department of Reproductive Biotechnology - Reproductive Biomedicine Research Center - Royan Institute for Biotechnology - ACECR, Isfahan , Ghaedi, Kamran Department of Cellular Biotechnology - Cell Science Research Center - Royan Institute for Biotechnology - ACECR, Isfahan - Department of Biology - Faculty of Sciences - University of Isfahan , Seifati, Morteza Department of Medical Biotechnology Research Center - Ashkezar Branch - Islamic Azad University - Ashkezar, Yazd , Nasr-Esfahani, Mohammad H. Isfahan Fertility and Infertility Center - Department of Reproductive Biotechnology - Reproductive Biomedicine Research Center - Royan Institute for Biotechnology - ACECR, Isfahan - Department of Cellular Biotechnology - Cell Science Research Center - Royan Institute for Biotechnology - ACECR, Isfahan
Abstract :
Purpose: The aim of this study was to evaluate expression of Post-Acrosomal WW Binding Protein (PAWP) in
infertile men with low and high fertilization post ICSI and also globozoospermic men.
Materials and Methods: Semen samples were collected from 18 infertile men with previously failed or low fertilization
(< 25%) post ICSI, 10 men with high fertilization (>50%) post ICSI, 15 globozoospermic men, and 21 fertile
individuals. Then, expression of PAWP was assessed at RNA with quantitative Real Time PCR.
Results: Relative expression of PAWP in sperm was significantly (P < .05) lower in infertile men with globozoospermia
(41.5 ± 5.7) or low fertilization rate (43.3±10.4) compared to fertile (138.8 ± 17.3)or men with high fertilization
(211.6 ± 75.6). In addition, a significant positive correlation (r = 0.628; P = .001) was observed between
percentage of fertilization with the relative expression of PAWP.
Conclusion: Considering solid recent evidences regarding PLCζ as the main sperm factor involved in oocyte activation,
therefore co-localization of PLCζ with PAWP in perinuclear theca may account for the above observation
and it is likely that PAWP may have other functions and/or it may assist PLCζ.
Keywords :
PAWP , fertilization , oocyte activation , globozoospermia
