Author/Authors :
Amouzeshi, Ahmad Surgery and Trauma Research Group - Department of Cardiovascular Surgery - Birjand University of Medical Sciences, Birjand, Iran , Moossavi, Zahra Medical School - Shiraz University of Medical Sciences, Shiraz, Iran , Javad Moosavi, Yoosef Birjand University of Medical Sciences, Birjand, Iran , Zardast, Mahmoud Birjand University of Medical Sciences, Birjand, Iran , Malekaneh, Mohammad Cellular and Molecular Research Center - Birjand University of Medical Sciences, Birjand, Iran , Esmaili, Sajad Birjand University of Medical Sciences, Birjand, Iran , Taneh, Abdolghader Birjand University of Medical Sciences, Birjand, Iran , Lotfi, Nasim Cellular and Molecular Research Center - Birjand University of Medical Sciences, Birjand, Iran , Moossavi, Maryam Birjand University of Medical Sciences, Birjand, Iran , Hoshyar, Reyhane Cellular and Molecular Research Center - Birjand University of Medical Sciences, Birjand, Iran
Abstract :
Background: Some plant extracts can prevent kidney stone formation in a dosedependent manner. In our study, we aimed to investigate the protective role of
Cannabis sativa aqueous extraction on calcium oxalate formation in ethylene glycolinduced rats.
Materials and Methods: To evaluate anti-urolithiasis, variations of the main risk factors
(citrate, phosphorous, calcium, and Ph) have been evaluated in 24-hour urine samples
of rats one day before the end of the experiment. Rats were divided into 4 groups: group 1 was administered regular drinking water; group 2 was administered 1% ethylene glycol in drinking water, group 3 was administered 100 mg/kg of Cannabis sativa extract for oral administration by gavage along with drinking water containing 1% ethylene glycol; and group 4 was administered 200 mg/kg of Cannabis sativa extract by gavage along with drinking water containing 1% ethylene glycol. Finally, histopathological slides from the kidney were also evaluated.
Results: Results showed that 100 mg/kg and 200 mg/kg doses of Cannabis sativa
extract significantly reduced the mentioned risk factors in comparison with ethylene
glycol-treated rats (p<0.05).
Conclusion: Histopathological slides showed reduced calcifications with extract
treatment at 200 mg/kg of Cannabis sativa. Thus, this antioxidant herb can prevent
kidney stone formation. These findings pave the way for new therapy.
Keywords :
Antioxidants , Cannabis sativa , Ethylene Glycol , Rats , Urate calcium oxalate stone
