Comparison of PLGA/Fibrin and PLGA/Hyaluronic Acid Scaffolds for Chondrogenesis of Human Adipose-Derived Stem Cells

Background and Aims:Tissue engineering is a relatively novel field that has been intensely developing during recent years and has shownto beexcessivelypromising when used for cartilage regeneration. Scaffolds represent important components for tissue engineering. Materials and Methods:The Poly Lactic-Co-Glycolic Acid (PLGA) impregnated with fibrin and hyaluronic acid (HA) produce hybrid scaffolds. human adipose-derived stem cells (hADSCs) were seeded in scaffolds and cultured in chondrogenic media. The viability of cells in different groups was assessed by MTT. The expression of chondrogenic related genes [Sox9, type II collagen (Col II), Aggrecan(AGG)]and type X collagen(Col X) was quantified by real-time polymerase chain reaction. Results:The results of the real-time PCR showed SOX9, AGGand Col Xgene expression in the control groups beingsignificantly lowerthan the other groups(p≤0.05). It alsodemonstrated Col IIgene expression in the control group beingsignificantly lower than the PLGA/Fibrin and PLGA/Fibrin/HA groups (p≤0.05). The Col Xgene expression of cells in PLGA/HA and PLGA/Fibrin/HA groups significantly decreased in comparison with the PLGA/Fibrin group (p≤0.05).Conclusions:These conclusions indicate that administration of PLGA/ Fibrin and PLGA/HA scaffolds, particularlyPLGA/Fibrin/HA, motivateschondrogenesis in hADSCs. This can be diminished by decreasing hypertrophic markers and increasing characteristic markers of hyaline cartilage.