Title of article :
Frequency of Chlamydia Trachomatis in Symptomatic and Asymptomatic Non-pregnant Women in Golestan Province
Author/Authors :
Okhli ، Samira Department of Microbiology - Islamic Azad University, Ashkezar Branch , Anvari ، Shaghayegh Department of Microbiology - Golestan University of Medical Sciences , Jamali ، Aylar Department of Microbiology - Laboratory Sciences Research Center - Golestan University of Medical Sciences , JavadKazemi ، Mohammad Department of Microbiology - Islamic Azad University, Ashkezar Branch
Abstract :
Background and Objective: Chlamydia trachomatis is one of the most common causes of genital infection in men and women. Genital chlamydial infections in women are clinically asymptomatic in 7080% of the cases; therefore, the lack of timely diagnosis and treatment leads to complications such as infertility and ectopic pregnancy. The aim of this study was to evaluate the frequency of chlamydial infection in symptomatic and asymptomatic women in the Golestan province. Methods: This crosssectional study was conducted on 150 cervical swab samples obtained from 150 women referred to the clinic, after obtaining written consent and completion of questionnaires. The swab samples were transferred to laboratory in phosphatebuffered saline solution and DNA extraction was carried out using phenolchloroform and boiling methods. The frequency of chlamydial infection was evaluated by PCR. Results: None of the tested samples were found as Chlamydiapositive. Conclusion: The findings require that some more extensive research with larger sample sizes and dispersed population be performed to determine the true prevalence. Considering the serious complications of chlamydial infections and its asymptomatic nature, a highly sensitive and specific method such as PCR should be used to detect Chlamydia. It is suggested that this method be used along with a complementary test to obtain the results that are more accurate. Furthermore, conducting simultaneous studies on other populations at risk will be very helpful in obtaining representable national data.
Keywords :
Chlamydia Trachomatis , PCR , Vaginal Swab.
Journal title :
Medical Laboratory Journal
Journal title :
Medical Laboratory Journal