Title of article
Antibiotic Resistance and Detection of femA Gene in Staphylococcus Aureus Isolates from Raw Milk
Author/Authors
Khodadadi ، Pouya Department of Microbiology - University, Masjed-Soliman Branch , Bizhan zadeh ، Mehdi Department of Medical Genetics - School of Medicine - Ahvaz Jundishapur University of Medical Sciences , Najafi ، Akram Department of Marine Microbiology - Persian Gulf Marine Biotechnology Medicine Research Center - Bushehr University of Medical Sciences , Zarinpour ، Vajiheh Department of Genetic - Faculty of Science - Islamic Azad University, Damghan Branch , Moshfe ، Abdolali Cellular and Molecular Research Center - Yasuj University of Medical Sciences , Ansari ، Hossein Department of Microbiology - Islamic Azad University, Masjed-Soliman Branch
From page
40
To page
45
Abstract
Background and Objective: Antibioticresistant Staphylococcus aureus strains have become a problem in treatment of infections caused by S. aureus. This study aimed to evaluate antibiotic resistance in S. aureus isolates from raw milk and detect femA gene in these isolates, as a confirmatory test for identification of S. aureus species. Methods: This crosssectional study was performed on 110 raw milk samples. After culture in Cooked Meat broth, presence of S. aureus in grown colonies was confirmed in accordance with Iranian National Standard, No. 1194. Antibiotic resistance was then evaluated according to guidelines recommenced by the Clinical Laboratory Standards Institute. FemAspecific polymerase chain reaction was performed on antibioticresistant strains using specific primers and standard strains to differentiate S. aureus from other species. Results: S. aureus were found in 43 (39.09%) of the 110 collected samples. Among these isolates, 79.07% and 76.75% were phenotypically resistant to penicillin and ceftazidime, respectively. In addition, the femA gene was detected in all isolates. Conclusion: The results of this study show a high prevalence of resistance to penicillin and ceftazidime among S. aureus strains isolated from raw milk.
Keywords
Staphylococcus aureus , Antibiotic Resistance , PCR.
Journal title
Medical Laboratory Journal
Journal title
Medical Laboratory Journal
Record number
2506436
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