Title of article :
In Vitro Effect of Some Medicinal Plants on Leishmania major Strain MRHO/IR/75/ER
Author/Authors :
Raeisi ، Mojtaba Food, Drug and Natural products Health Research Center - Golestan University of Medical Sciences , Mirkarimi ، Kamal Department of Public Health - Management and Social Development Research Center, School of Health - Golestan University of Medical Sciences , Jannat ، Behrooz Halal Research Center of IRI - Food and Drug Administration , Rahimi Esboei ، Bahman Department of Parasitology and Mycology - Faculty of Medicine - Islamic Azad University, Tonekabon Branch , Pagheh ، Abdol Sattar Infectious Disease Research Center - Birjand University of Medical Sciences , Mehrbakhsh ، Zahra Department of Biostaticstics - School of Health - Hamadan University of Medical Sciences , Ghaffarifar ، Fatemeh Department of Parasitology - Faculty of Medical Sciences - Tarbiat Modares University , Jorjani ، Oghlniaz Laboratory Sciences Research Center - Golestan University of Medical Sciences , Foroutan ، Masoud Department of Parasitology - Faculty of Medical Sciences - Tarbiat Modares University
Abstract :
Background and objectives: Leishmaniasis is a tropical disease caused by protozoan parasites from the genus Leishmania. In this study, we aimed at investigating the in vitro anti-leishmanial effect of essential oils of Rosmarinus officinalis, Mentha pulegium, Foeniculum vulgare, Lippia citriodora and Pelargonium graveolens. Methods: The essential oils were prepared from freshly dried and powdered plants with steam-distilled water. Iranian strain of Leishmania promastigotes was cultured in RPMI medium and the inhibitory effects of different concentrations (25, 32, 62.5, 125, 250, 500 and 1000 μg/ml) of the essential oils were investigated at 24, 48 and 72 hours. The number of live parasites before and after treatment with the essential oils was counted by trypan blue 10% staining and using neobar lam. Results: The essential oils significantly decreased the number of promastigotes in a dose-dependent manner (P 0.05). However, the inhibitory effects of F. vulgare and R. officinalis essential oils were more profound compared to other essential oils. Moreover, concentrations of 500 and 1000 μg/ml of these two essential oils exerted equal and more antileishmanial potency compared to glucantime, the first-line drug used for treatment of leishmaniasis. Conclusion: Based on the results, it is recommended to evaluate the in vivo anti-leishmanial effects of the tested essential oils, particularly F. vulgare and R. officinalis.
Keywords :
Rosmarinus officinalis , Mentha pulegium , Foeniculum vulgare , Lippia citriodora , Pelargonium graveolens , Leishmania
Journal title :
Medical Laboratory Journal
Journal title :
Medical Laboratory Journal
