Title of article
Cloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System
Author/Authors
Borhani ، K Department of Virology - School of Medical Sciences - Tarbiat Modares University , Bamdad ، T Department of Virology - School of Medical Sciences - Tarbiat Modares University , Ajorloo ، M Department of Virology - School of Medical Sciences - Tarbiat Modares University , Mozhgani ، SHR Human Rabies Vaccine Laboratory - Institute of Iran , Miandehi ، N Viral Vaccines Production Department - Production and Research Complex Pasteur, - Institute of Iran , Moradi-Joshaghan ، A Viral Vaccines Production Department - Production and Research Complex Pasteur - Institute of Iran , Gholami ، AR Human Rabies Vaccine Laboratory, Viral Vaccines Production Department - Production and Research Complex Pasteur - Institute of Iran
From page
8
To page
11
Abstract
Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verification of the cloning, the recombinant plasmid was transfected into BSR cell line (a clone of BHK21 cell), and its expression was detected by RTPCR. Results: The authenticity of the recombinant plasmid pcDNA3.1(+)Gp has been confirmed by a quick check method and restriction endonuclease digestion analysis, and after transfection into eukaryotic cells, the presence of mRNA transcripts was verified by reverse transcriptasepolymerase chain reaction (RTPCR). Conclusion: This study demonstrated that the construction of eukaryotic expression plasmid for rabies virus glycoprotein is possible. Nevertheless, more work is necessary to develop this kind of vaccine for final use.
Keywords
Rabies virus , glycoprotein , DNA vaccine , Reverse Transcriptase , Polymerase Chain Reaction (RT , PCR)
Journal title
Iranian Journal of Virology
Journal title
Iranian Journal of Virology
Record number
2507547
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