Adenovector Mediated Inflammation in Mice Liver Boosted by Hydrodynamic Injection

Background and Aims: Adenovector gene transfer induces inflammatory response that finally leads to vector removal from delivered site. The effect of hydrodynamic preinjection on Adenovector mediated liver inflammation remained elusive. Materials and Methods: Different mice groups were pretreated by hydrodynamic saline, dexamethasone or nothing prior to AdenoLuciferase administration. Their samples were collected in 18 hours later and serum IL1 beta; level as a marker of inflammation was quantified. Also, liver histological assessment was performed to score the inflammatory grade. Results and Conclusion: In spite of dexamethasone group, both hydrodynamic/AdLuc and AdLuc receiving groups induced the expression of IL1 beta; cytokine (p 0.05). The pathological views also indicated more inflammation and necrosis inside the different zones of liver tissue in these two adenovector injected groups. These results emphasized that Adenovector served as a suitable inflammatory model and hydrodynamic injection enhances this kind of inflammation.