Author/Authors :
Mahdizadeh, Shadi Department of Cell and Molecular Biology - Kish International Campus - University of Tehran, Kish , Karimi, Gholamreza Medical Toxicology Research Center - School of Pharmacy - Mashhad University of Medical Sciences, Mashhad , Behravan, Javad Biotechnology Research Center - School of Pharmacy - Mashhad University of Medical Sciences - P. O. Box 91775-1365, Mashhad - Department of Pharmaceutical Biotechnology - School of Pharmacy - Mashhad University of Medical Sciences, Mashhad , Arabzadeh, Sepideh Biotechnology Research Center - School of Pharmacy - Mashhad University of Medical Sciences - P. O. Box 91775-1365, Mashhad , Lage, Hermann Institute of Pathology - Charite University - Campus Mitte - Humboldt - Berlin - Germany , Kalalinia, Fatemeh Medical Genetic Research Center - Mashhad University of Medical Sciences, Mashhad - Biotechnology Research Center - School of Pharmacy - Mashhad University of Medical Sciences - P. O. Box 91775-1365, Mashhad
Abstract :
Background: Crocin, one of the main constituents of saffron extract, has numerous biological effects such as anti-cancer effects. Multidrug resistance-associated proteins 1 and 2 (MRP1 and MRP2) are important elements in
the failure of cancer chemotherapy. In this study we aimed to evaluate the effects of crocin on MRP1 and MRP2
expression and function in human ovarian cancer cell line A2780 and its cisplatin-resistant derivative A2780/RCIS cells.
Methods: The cytotoxicity of crocin was assessed by the MTT assay. The effects of crocin on the MRP1 and MRP2
mRNA expression and function were assessed by real-time RT-PCR and MTT assays, respectively.
Results: Our study indicated that crocin reduced cell proliferation in a dose-dependent manner in which the
reduction in proliferation rate was more noticeable in the A2780 cell line compared to A2780/RCIS. Crocin
reduced MRP1 and MRP2 gene expression at the mRNA level in A2780/RCIS cells. It increased doxorubicin
cytotoxicity on the resistant A2780/RCIS cells in comparison with the drug-sensitive A2780 cells.
Conclusion: Totally, these results indicated that crocin could suppress drug resistance via down regulation of MRP transporters in the human ovarian cancer resistant cell line.
Keywords :
Crocin , Multidrug resistance , MRP1 , MRP2 , A2780 , A2780/RCIS
