Author/Authors :
Mokhtari, Tahmineh Department of Anatomy - School of Medicine - Tehran University of Medical Sciences, Tehran , Akbari, Mohammad Department of Anatomy - School of Medicine - Tehran University of Medical Sciences, Tehran , Malek, Fatemeh Department of Anatomy - School of Medicine - Tehran University of Medical Sciences, Tehran , Ragerdi Kashani, Iraj Department of Anatomy - School of Medicine - Tehran University of Medical Sciences, Tehran , Rastegar, Tayebeh Department of Anatomy - School of Medicine - Tehran University of Medical Sciences, Tehran , Noorbakhsh, Farshid Department of Immunology - School of Medicine - Tehran University of Medical Sciences, Tehran , Ghazi-Khansari, Mahmoud Department of Pharmacology - School of Medicine - Tehran University of Medical Sciences , Attari, Fatemeh Department of Neuroscience - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences , Hassanzadeh, Gholamreza Department of Anatomy - School of Medicine - Tehran University of Medical Sciences, Tehran
Abstract :
Background: Ischemic stroke is a common leading cause of death and disability with lack of effective therapies. In
this study, T3 was intra-ventricularly injected to evaluate gene expression and protein concentration of and brainderived neurotrophic factor (BDNF) and Glial cell-derived neurotrophic factor (GDNF) in hippocampal CA1 region in
rat model of brain ischemia/reperfusion (I/R).
Methods: In this study, transient middle cerebral artery occlusion (tMCAo) was used as model of ischemic brain
stroke. Rats were randomly divided in four groups of Co, Sh, tMCAo and tMCAo + T3. Then, a single dose of intraventricular T3 was administered via a Hamilton syringe. Passive avoidance test was used as behavioral
investigations. After 21 days, the animals were sacrificed and their brains were used for molecular and
histopathological studies.
Results: T3 significantly improved the learning and memory compared with tMCAo group according to Morris
water maze findings (P < 0.05). Step-through latency (STL) significantly decreased in tMCAo group (P < 0.05). There
were significant increase in the STL of T3 group compared with tMCAo group (P < 0.05).A significant reduction in
BDNF mRNAs and protein levels were observed in the tMCAo compared with Co and Sh group (P < 0.05). A
significant increase of BDNF and GDNF mRNAs and proteins was recorded in tMCAo + T3 group compared with Co,
Sh and tMCAO groups (P < 0.05).
Conclusions: The results of current study demonstrated that T3 had therapeutic effects on cerebral ischemic stroke by increasing the neurotrophic factors (BDNF, GDNF) in CA1 region of hippocampus.
Keywords :
Triiodothyronine , BDNF , GDNF , CA1 , Stroke
