Title of article :
First report of Tasmanian sheep strain (G2) genotype isolated from Iranian goat using the high resolution melting (HRM) analysis
Author/Authors :
Hosseini-Safa, Ahmad Department of Medical Parasitology and Mycology - School of Public Health - Tehran university of Medical Sciences, Iran , Mohaghegh, Mohammad Ali Department of Parasitology and Mycology - School of Medicine - Isfahan University of Medical Science, Iran , Pestechian, Nader Department of Parasitology and Mycology - School of Medicine - Isfahan University of Medical Science, Iran , Ganji, Maryam Department of Immunology - School of Medicine - Isfahan University of Medical Sciences, Iran , Mohammadi, Rasoul Department of Parasitology and Mycology - School of Medicine - Isfahan University of Medical Science, Iran , Mahmoudi Lamouki, Reza Proteomics Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Rostami-Nejad, Mohammad Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Aim: The present study was aimed to evaluate E. granulosus genotypes isolated from goats using HRM analysis in Isfahan province.
Background: Cystic echincoccosis, so-called hydatidosis, is widespread infection caused by the larval stage of Echinococcus
granulosus. This is an important zoonotic disease worldwide, especially in the developing countries such as Iran. To date, molecular
studies mainly based on the mitochondrial DNA sequences have identified distinct genotypes termed G1-G10 which can differ in
some characteristics such as the growth and infectivity to different intermediate hosts or the survival rate in the definitive hosts that are
important for the development of control strategies.
Methods: From August to December 2014, 1341 goats were investigated and hydatid cysts were collected from the liver and lungs
of 43 infected goats in Isfahan province abattoirs, Isfahan, Iran. Total genomic DNA was extracted from each sample, amplified for
the presence of polymorphism of mitochondrial gene coding for cytochrome c oxidase subunit 1 (CO1), using high resolution melting
curve (HRM) method.
Results: the results of HRM analysis using the sequence of CO1 gene for 43 Echinococcus granulosus isolates from goats showed
31, 2 and 10 isolates were identified as G1, G2, and G3 genotypes, respectively.
Conclusion: G1 is the predominant genotype in the isolated goat samples in Isfahan province, and the presence of G2 strain was
reported for the first time in goat in Iran.
Keywords :
Echinococcus granulosus , G2 genotype , HRM , goat , Iran
Journal title :
Gastroenterology and Hepatology From Bed to Bench
