Author/Authors :
Behboudi Farahbakhsh, Faegheh Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Nazemalhosseini Mojarad, Ehsan Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Azimzadeh, Pedram Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Goudarzi, Faezeh Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Mohammad Alizadeh, Amir Houshang Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Haghazali, Mehrdad Behbood Gastroenterology and Liver Diseases Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Mahmoudi Lamoki, Reza Proteomics Research Center - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Asadzadeh Aghdaei, Hamid Gastroenterology and Liver Diseases Research Center - Research Institute for Gastroenterology and Liver Diseases - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
Aim: Our aim was to determine the association between TGF-β 1 polymorphisms at position-509 C>T (rs1800469) and +915 G>C (rs1800471) and pancreatic cancer susceptibility in Iranian population. Background: Ninety percent of pancreatic cancer patients have less than 5-year overall survival and approximately 50% of cases were diagnosed with metastasis in the time of admission. Previous evidences have demonstrated the strong association between TGF-β 1 variations and cancer susceptibility so far. Methods: A total of 78 patients with pancreatic cancer and 94 healthy controls were enrolled in this case control study from 2007-2012. Genomic DNA was isolated from peripheral blood samples according to phenol chloroform extraction. The genotypes of TGF-β 1 rs rs1800469 and rs1800471 were determined using the polymerase chain reaction-restriction fragment length polymorphism method. Results: The mean age of cases and the control group were 64. 50 ± 13. 718 and 40. 12 ± 16. 001, respectively. For polymorphism-509 C>T, the frequency of TT genotype were 31 (33. 0), CT, 47(50) and CC, 16 (17) in control and 19 (24. 4), 45 (57. 7) and 14 (17. 9) in cases respectively. In position +915 G>C, the frequency of GG genotype was 84 (89. 4) and GC, 10 (10. 6) in control and 71 (91. 0) and 7 (9) in cases, respectively. We did not observe any significant differences in the genotype and allele frequencies of the TGF-β 1-509 C>T (rs1800469) and codon +915 G>C (rs1800471) between the two study groups (P>0. 05). Conclusion: we found that TGF-β 1 gene polymorphisms rs1800469 and rs1800471 might not play a role in pancreatic cancer susceptibility in Iranian population.
Keywords :
TGF-β1 , Transforming growth factor beta 1 , dNTP , deoxyribonucleotide triphosphate
