Title of article :
Evaluation of Lomustine-Loaded Iron Nanoparticles on Caspase-6 Gene Expression and Cell Viability in U87Mg Cell Line
Author/Authors :
Sharifian Dastjerdi ، Zeinolabedin Department of Anatomical Sciences - Medical School, Molecular Medicine Research Center, Hormozgan Health Institute - Hormozgan University of Medical Sciences , Kargar Abargouei ، Elyas Department of Anatomical Sciences - Medical School, Molecular Medicine Research Center, Hormozgan Health Institute - Hormozgan University of Medical Sciences , Jafari ، Salman Department of Radiology Technology - School of Paramedicine - Hamadan University of Medical Sciences , Eftekhar ، Ebrahim Molecular Medicine Research Center, Hormozgan Health Institute - Hormozgan University of Medical Sciences , Pourentezari ، Majid Department of Biology and Anatomical Sciences - Medical School - Shahid Sadoughi University of Medical Sciences , Zamani Rarani ، Fahimeh Department of Anatomical Sciences - School of Medicine - Isfahan University of Medical Sciences , Afzalipour ، Reza Department of Radiology - Molecular Medicine Research Center, Hormozgan Health Institute, Faculty of Para-Medicine - Hormozgan University of Medical Sciences , Mohajer Ansari ، Javad Department of Anatomical Sciences - Medical School - Hormozgan University of Medical Sciences , Zamani Rarani ، Mohammad Department of Anatomical Sciences - Medical School, Molecular Medicine Research Center, Hormozgan Health Institute - Hormozgan University of Medical Sciences
Abstract :
Background: Every year, many people around the world die from cancers. Among all types of cancers, brain cancer has been recognized as one of the most deadly cancers due to the late detection and limitations of current therapies, and thus it remains an unresolved problem. Glioblastoma occurs in different parts of the central nervous system and is one of the most important causes of cancer death in people. In addition, there are many problems for the treatment of cancer cells. One of the limiting factors is the resistance of cancer cells to chemotherapy drugs. In this regard, the use of nanoparticles (NPs) is an effective method for overcoming this problem. Materials and Methods: In this study, iron oxide-NPs were synthesized and loaded on the folic and lomustine. Further, the size and morphology of NPs were assessed by transmission electron microscopy, X-ray photoelectron spectroscopy, and dynamic light scattering. Then, the U87-MG cell line was cultured in the Dulbecco’s Modified Eagle Medium and treated with nano, nano-folic, nano-lomustine (LUM), LUM, and complex, followed by evaluating 50% inhibitory concentration, tetrazolium assay, and caspase-6 activity. Results: Our results showed that cell viability decreased in LUM container groups by increasing the incubation time. Based on the caspase-6 activity analysis, the mortality rate increased in LUM container groups after 3 days. These findings indicated that LUM, complex, and nano-LUM increase cell death in U87MG. Conclusion: Finally, the results suggested that LUM in NPs could be applied as a safer form of drug delivery for targeting cancer.
Keywords :
Targeted therapy , Nanoparticle , Apoptosis , Caspase , U87MG
Journal title :
Disease and Diagnosis
Journal title :
Disease and Diagnosis
