Author/Authors :
Eleftheriadis, Theodoros Department of Nephrology - Medical School - University of Thessaly, Larissa, Greece , Pissas, Georgios Department of Nephrology - Medical School - University of Thessaly, Larissa, Greece , Antoniadi, Georgia Department of Nephrology - Medical School - University of Thessaly, Larissa, Greece , Tsogka, Konstantina Department of Nephrology - Medical School - University of Thessaly, Larissa, Greece , Makri, Panagiota Department of Nephrology - Medical School - University of Thessaly, Larissa, Greece , Liakopoulos, Vassilios Department of Nephrology - Medical School - University of Thessaly, Larissa, Greece , Stefanidis, Ioannis Department of Nephrology - Medical School - University of Thessaly, Larissa, Greece
Abstract :
Hemodialysis patients suffer from susceptibility to infections.
Inflammation upregulates indoleamine 2,3-dioxygenase (IDO) in the
antigen-presenting cells, which suppresses T-cell function. Plasma IDO
activity or protein expression is increased in hemodialysis patients
and is associated with immune disturbances. This observation,
however, does not consider many factors, importantly the source
of IDO, which has to be the antigen-presenting cells in order IDO
to exert its immunosuppressive effect in the microenvironment
of the immune response. In this study, monocytes were isolated
from 30 hemodialysis patients and 20 healthy volunteers and IDO
was assessed by Western blotting. The IDO level in the monocytes
of hemodialysis patients was significantly, almost 3-fold, higher
than in the monocytes of healthy volunteers. This localization
enables IDO to exert its immunosuppressive effect and supports
conclusions of previous studies that used more indirect methods
for assessing the role of this enzyme in the context of the immune
response in hemodialysis patients.
Keywords :
immune response , monocytes , 3-dioxygenase , indoleamine 2 , hemodialysis
