Author/Authors :
Tezcan, Orhan Department of Cardiovascular Surgery - Medical School of Dicle University, Diyarbakir, Turkey , Caliskan, Ahmet Department of Cardiovascular Surgery - Medical School of Dicle University, Diyarbakir, Turkey , Demirtas, Sinan Department of Cardiovascular Surgery, Medical School of Dicle University, Diyarbakir, Turkey , Yavuz, Celal Department of Cardiovascular Surgery, Medical School of Dicle University, Diyarbakir, Turkey , Kuyumcu, Mahir Department of Anesthesiology - Medical School of Dicle University, Diyarbakir, Turkey , Nergiz, Yusuf Department of Histology and Embryology - Medical School of Dicle University, Diyarbakir, Turkey , Guzel, Abdulmenap Department of Anesthesiology - Medical School of Dicle University, Diyarbakir, Turkey , Karahan, Oğuz Department of Cardiovascular Surgery - Medical School of Dicle University, Diyarbakir, Turkey , Ari, Şeyhmus Department of Ophthalmology - Medical School of Dicle University, Diyarbakir, Turkey , Soker, Sevda Department of Histology and Embryology - Medical School of Dicle University, Diyarbakir, Turkey , Yalinkilic, İbrahim Department of Medical Biology - Medical School of Dicle University, Diyarbakir, Turkey , Turkdogan, Kenan Ahmet Department of Emergency Medicine - Medical School of Adnan Menderes University, Diyarbakir, Turkey
Abstract :
Introduction. Hyperbaric oxygen (HBO) treatment is steadily
increasing as a therapeutic modality for various types of diseases.
Although good clinical outcomes were reported with HBO treatment
for various diseases, the multisystemic effects of this modality are
still unclear. This study aimed to investigate the renal effects of
HBO experimentally.
Materials and Methods. Fourteen New Zealand White rabbits
were divided into 2 groups randomly as the control group and
the study group. The study group received HBO treatment for
28 days (100% oxygen at 2.5 atmospheres for 90 minutes daily)
and the control group was used to obtain normal renal tissue of
the animal genus. After the intervention period, venous blood
samples were obtained, and renal tissue samples were harvested
for comparisons.
Results. Normal histological morphology was determined with
Masson trichrome staining and periodic acid-Schiff staining in the
control group. Atrophic glomerular structures, vacuolated tubule
cells, and degeneration were detected in the renal samples of the
study group with Masson trichrome staining. Additionally, flattening
was observed on the brush borders of the proximal tubules, and
tubular dilatation was visualized with periodic acid-Schiff staining.
The histopathologic disruption of renal morphology was verified
with detection of significantly elevated kidney function laboratory
biomarkers in the study group.
Conclusions. Our findings suggests that HBO has adverse effects
on renal glomerulus and proximal tubules. However, the functional
effects of this alteration should be investigated with further studies.
