Prognostic Impact of Circulating CD28 Negative Suppressor T Cells and Memory B Cells on Treatment Outcomes of Patients with Breast Cancer

Background: Circulating lymphocytes can put up understanding of the interactions between tumor cells and lymphocytes infiltrating the tumor microenvironment. Objective: In this sense, the prognostic value of the inflammatory cell in breast cancer (BC) peripheral blood needs to be evaluated; this would suggest that estimation and evaluation of these circulating lymphocytes could provide a prognostic value in BC. Methods: The present study was a prospective case-control study, 30 Patients with operable breast cancer; any T, any N, M0, treated surgically with either modified radical mastectomy or breast conservative surgery, and 20 healthy controls were included. For detection of lymphocyte subsets in peripheral blood; Fluorochrome-labeled monoclonal primary antibodies were used, the cells were analyzed by FACSCalibur flow cytometry with Cell Quest software, then patients were treated with chemotherapy, 3DCRT, and hormonal treatment, and followed up to determine relapses and recurrence-free survival (RFS). Results: Significant differences of different cell types B, T, NK, NKT, and CD28- T between BC patients and controls, significant differences of CD8+CD28-cells among different biologic subtypes, negative correlations between CD8+CD28-cells and memory B cells, and RFS. Significant differences of naïve B cells among different grades of BC, and negative correlation between B cells and NKT cells. Conclusion: NK, NKT, lymphocytes, and CD28-Tcells significantly differed between healthy controls and BC patients; also, different B cells were associated with good response to treatment while CD28-cells were associated with shorter RFS.