Author/Authors :
Zhou, Yi Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Li, Bao-ying Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Li, Xiao-li Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Wang, Ya-juan Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Zhang, Zhen Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Pei, Fei Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Wang, Quan-zhen Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Zhang, Jun Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Cai, Ya-wei Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Cheng, Mei Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China , Gao, Hai-qing Department of Geriatrics - Qi-Lu Hospital of Shandong University - Key Laboratory of Cardiovascular Proteomics of Shandong Province, Jinan, Shandong, China
Abstract :
Grape seed procyanidin B2 (GSPB2) exerts a variety of potent
protective pharmacological effects on diabetic complications. The
renal protective effects of GSPB2 and the target protein mimecan
regulated by GSPB2, discovered in a previous quantitative proteomic
analysis, were assessed in mice with diabetic nephropathy Twentyfour
db/db mice were divided into 2 groups of the vehicle-treated
and GSPB2-treated (30 mg/kg/d) diabetic groups. All animals
were observed for 10 weeks. Treatment with GSPB2 resulted in
an improvement in body weight increase and serum levels of
triglyceride, total cholesterol, advanced glycation end products,
and urinary albumin excretion in comparison with the vehicletreated
diabetic mice (P < .05), although these levels were still
higher than those in the control group. Treatment with GSPB2
significantly reduced the extent of glomerular basement membranes
thickening, mesangial expansion, and glomerular area as well.
Mimecan protein expressions in diabetes mellitus were decreased
approximately by 28% when compared with those in the control
group (P < .05), and restored remarkably after GSPB2 treatment
(P < .05). The expression of nuclear factor-κB (NF-κB) p65 in nuclear
extracts, markedly higher in the diabetic mice than in the controls,
was significantly suppressed by GSPB2. The findings of this study
revealed that mimecan might become a new therapeutic target
in the future and indicated that GSPB2 had beneficial effects not
only on oxidative stress, but also on renal fibrosis, particularly in
the diabetic kidney.
