Author/Authors :
LIU, Yibing Department of Medical Oncology - Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China , MENG, Qingju Department of Orthopedics - The First Hospital of Xingtai, Xingtai, Hebei Province, China , JING, Li Department of Medical Oncology - Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China , FENG, Li Department of Medical Oncology - Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China , ZHOU, Zhiguo Department of Medical Oncology - Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China , NI, Zhiyu School of Basic Medical Science - Hebei University - The Affiliated Hospital of Hebei University, Bao ding, Hebei Province, China
Abstract :
Background: To study the anti-cancer effect of isoalantolactone, a sesquiterpene lactoneisolated from the roots of Inula heleniumon human gynecologic cancer cells.
Methods: A structure-activity relationship experiment was designed to identify the functional moiety of isoalantolactone for its significant anti-cancer activity. Five gynecologic cancer cell lines were treated with isoalantolactone. Cell proliferation was determined by MTT assay in vitro and cell apoptosis by flow cytometry.
Results: We found isoalantolactone strongly inhibited the cell proliferationofHEC-1, HAC-2, HOC-21, and HeLa cells. Its inhibitory effect was comparable to that of well-known chemotherapeutic agents, cisplatin and taxol. Furthermore, isoalantolactone induced apoptosis in HeLa cells via caspase. On the contrary, its 11, 13-dihydro derivatives had much weaker anti-proliferative activities than the parent compound.
Conclusion: Isoalantolactone exhibited strong anti-proliferative activities and apoptosis-inducing effects on gynecologic cancer cells. The 11, 13-dehydro lactone moiety was critical for its anti-proliferative activity.
Keywords :
Apoptosis , Gynecologic cancer , Inulahelenium , Isoalantolactone , Proliferation
