Author/Authors :
Kazemi, Mohammad Department of Anatomical Sciences - Rafsanjan University of Medical Sciences, Rafsanjan, I.R. Iran , Taghavi, Mohammad-Mohsen Department of Anatomical Sciences - Rafsanjan University of Medical Sciences, Rafsanjan, I.R. Iran , Shabanizadeh, Ahmad Department of Anatomical Sciences - Rafsanjan University of Medical Sciences, Rafsanjan, I.R. Iran , Taghipour, Zahra Department of Anatomical Sciences - Rafsanjan University of Medical Sciences, Rafsanjan, I.R. Iran , Kaeidi, Ayat Physiology-Pharmacology Research Center - Rafsanjan University of Medical Sciences, Rafsanjan, I.R. Iran , Shariati-Kohbanani, Mahdi Department of Anatomical Sciences - Rafsanjan University of Medical Sciences, Rafsanjan, I.R. Iran
Abstract :
Background and purpose: Natural products are used to improve the damage caused by harmful reagents in various pathological situations. This study investigated the effect of grape sap as a natural product with antioxidant properties on follicle cell proliferation in bleomycin (as a chemotherapy agent with toxic effects on hair growth) treated rats skin.
Experimental approach: The bleomycin treated rats were administrated grape sap. Wingless/integrated (wnt) and β-catenin gene expression as follicle proliferative markers were evaluated using real-time polymerase chain reaction. Furthermore, histological factors and total antioxidant capacity were evaluated.
Findings / Results: The data showed that, grape sap increased the number of anagenic hair follicle in grape sap (100 mg/kg) group (P < 0.001), sebaceous glands (P < 0.001), blood vessel density (P < 0.001), and hair growth length (P < 0.001). Also, wnt and β-catenin gene expression was elevated. The data showed that wnt and β-catenin gene expression were elevated in grape sap treated animals versus bleomycin group (P < 0.01 and 0.001, respectively).
Conclusion and implications: Our finding showed that grape sap can be effective in increasing hair growth a gains bleomycin toxic effects on skin hair growth.
Keywords :
Antioxidant capacity , β-catenin , Grape sap , Hair follicle , wnt , Rat