Author/Authors :
Mirhafez, Reza Department of Basic Medical Sciences - Neyshabur University of Medical Sciences , Avan, Amir Department of Modern Science and Technologies - and Biochemistry of Nutrition Research Center , Pasdar, Alireza ivision of Applied Medicine - Medical School, University of Aberdeen - UK , Khatamianfar, Sara Department of Modern Science and Technologies - and Biochemistry of Nutrition Research Center , Hosseinzadeh, Leila Department of Modern Science and Technologies - and Biochemistry of Nutrition Research Center , Ganjali, Shiva Department of Modern Science and Technologies - and Biochemistry of Nutrition Research Center , Movahedi, Ali Department of Basic Medical Sciences - Neyshabur University of Medical Sciences , Pirhoushiaran, Maryam VU University Medical Center, Amsterdam - The Netherlands , Gómez Mellado, Valentina VU University Medical Center, Amsterdam - The Netherlands , Rosace, Domenico University of Bologna - Bologna - Italy , Van Krieken, Anne Peter MacCallum Centre - St Andrew's Place - Melbourne - Australia , Nohtani, Mahdi Department of Modern Science and Technologies - and Biochemistry of Nutrition Research Center , Ferns, Gordon A Brighton & Sussex Medical School, Division of Medical Education - UK , Ghayour-Mobarhan, Majid Department of Modern Science and Technologies - and Biochemistry of Nutrition Research Center
Abstract :
Metabolic syndrome (MetS) is characterized by a cluster of cardiovascular risk factors that include: abdominal obesity, dyslipidaemia, hypertension, insulin resistance and impaired glucose tolerance. Recent genome wide association studies have identified several susceptibility regions involved in lipid metabolism that are also associated with MetS. We have explored the association of 9 genetic polymorphisms involved in lipid metabolism and hypertension, including: MTHFR C677T, SELE L554F, FGB - 455G>A, GNB3 C825T, ZNF259 C>G, PSRC-1 A>G, CETP I405V, LPL S447X and LPA C>T in 97 subjects with mets and 96 individuals without MetS who were recruited randomly from Mashhad stroke and heart atherosclerotic disorder (MASHAD) study using a stratified cluster random sampling technique. Anthropometric parameters and biochemical measurements were determined in all the subjects. Genotyping was carried out followed by univariate and multivariate analyses. The subjects with MetS had a higher triglyceride and lower HDL- C. CG+ GG genotypes of ZNF259 polymorphism (rs964184 C>G) and TT+CT genotypes of MTHFR C677T (rs1801133) were associated with MetS, and individuals carrying the G allele for ZNF259 or the T allele for MTHFR polymorphisms were associated with MetS (e.g, odds ratio (OR) for CG+GG genotypes vs. CC wild type: 2.52, CI=1.33-4.77; P=0.005). However, after multiple comparison adsustments, this relationship remained significant only for CG+ GG genotypes of ZNF259 polymorphism. Moreover, the ZNF259 CG+ GG genotypes were associated with increased serum concentrations of triglycerides and LDL-C, compared to the wild type. These data support the necessity of further studies in larger multicenter settings.
