Author/Authors :
Rasekh, Hosein Department of Horticultural Sciences - Yasooj Branch - Islamic Azad University, Yasooj , Hoseini Farahi, Mehdi Department of Horticultural Sciences - Yasooj Branch - Islamic Azad University, Yasooj , Mehrabani, Davood Stem Cell Technology Research Center - Department of Pathology - Shiraz University of Medical Sciences, Shiraz - Burn and Wound Healing Research Center - Department of Pathology - Shiraz University of Medical Sciences, Shiraz - Comparative and Experimental Medicine Center - Shiraz University of Medical Sciences, Shiraz - Department of Pathology - University of Alberta - Edmonton - AB - Canada , Massoumi, Jalil Nutrition and Food Sciences Research Center - Department of Nutrition - School of Nutrition and Food Sciences - Shiraz University of Medical Sciences , Ramzi, Mani Hematology Research Center - Shiraz University of Medical Sciences , Acker, Jason P. Centre for Innovation - Canadian Blood Services - Edmonton - AB - Canada - Department of Pathology - University of Alberta - Edmonton - AB - Canada
Abstract :
BACKGROUND
Feijoa is widely used in medicine due to their anti-inflammatory, antioxidant, antimicrobial and antitumor properties. The current
investigation studied the proliferative and regenerative effect of
acetonic extract of Feijoa sellowiana on stem cells.
METHODS
Acetone extract of Feijoa was prepared using percolator and rotary
machines. Human bone marrow stem cells (hBMSCs) were used
as experimental in vitro model and characterized morphologically,
by flowcytometry, and differentiation properties. The toxicity
of the extract on hBMSCs was determined by MTT assay. The
viability and growth kinetics of hBMSCs treated to Feijoa was
determined. Real time PCR was used for changes in expression
of proliferative and apoptotic genes on day 7th.
RESULTS
MTT assay demondtrated that Feijoa at doses less than 200 ng/ml
did not show any cytotoxic effect on hBMSCs and increased the
cell proliferation until day 3rd followed by a non-significant slow
decreasing trend until day 7th. Population doubling time (PDT)
showed a decline until day 3rd followed by an increase until day
7th. A significant rise in expression of Bax and decline in Bcl-2
expression were noted on day 7th.
CONCLUSION
The modulatory activity of Feijoa may be responsible for its
increasing effect on cell proliferation till day 3rd. Therefore, when
faster proliferation during a shorter time period is targeted, Feijoa
can be safely added to the culture media in the first three days.
Keywords :
Feijoa sellowiana , Bone marrow stem cells , Growth kinetics , Apoptosis
