Author/Authors :
Bahrami, Gholamreza Medical Biology Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Hosseinzadeh, Leila Pharmaceutical Sciences Research Center - Faculty of Pharmacy - Kermanshah University of Medical Sciences, Kermanshah, Iran , Azadid, Zahra Students research committee - Faculty of Pharmacy - Kermanshah University of Medical Sciences, Kermanshah, Iran , Mohammadi, Bahareh Medical Biology Research Center - Kermanshah University of Medical Sciences, Kermanshah, Iran , Mahdiyan, Zahra Students research committee - Faculty of Pharmacy - Kermanshah University of Medical Sciences, Kermanshah, Iran , Hajialyani, Marziyeh Pharmaceutical Sciences Research Center - Faculty of Pharmacy - Kermanshah University of Medical Sciences, Kermanshah, Iran , Farzaei, Mohammad Hosein School of Pharmacy - Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract :
This study evaluated the anti-atherosclerosis effect of aqueous extract
and polysaccharide-enriched fraction of Prosopis farcta on adhesion and
inflammatory cascades in vascular endothelial cells and related
molecular mechanisms. Cellular toxicities of LPS, plant extract and
polysaccharide-enriched fraction were analyzed in vitro using the MTT
method. The ROS accumulation, mRNA expression of ICAM1 and VCAM1,
and COX activity were measured in HUVEC cells. These results revealed
a new underlying mechanism for anti-atherosclerosis effect of P. farcta
and polysaccharide-enriched fraction by attenuating inflammatory
cascade mediated by COX expression, modulating expression of celladhesion
molecules including VCAM-1 and ICAM-1, and diminishing
oxidative stress agents.
Keywords :
Prosopis Farcta , LPS , HUVEC , Cytotoxicity , Adhesion Molecules , Atherosclerosis
