Author/Authors :
Behdani, Mahdi Biotechnology Research Center - Venom and Biotherapeutics Molecules Lab - Pasteur Institute of Iran - Tehran, Iran , Shokrollahi, Narjes Biotechnology Research Center - Venom and Biotherapeutics Molecules Lab - Pasteur Institute of Iran - Tehran, Iran , Shahbazzadeh, Delavar Biotechnology Research Center - Venom and Biotherapeutics Molecules Lab - Pasteur Institute of Iran - Tehran, Iran , Pooshang-Bagheri, Kamran Biotechnology Research Center - Venom and Biotherapeutics Molecules Lab - Pasteur Institute of Iran - Tehran, Iran , Habibi-Anbouhi, Mahdi National Cell Bank of Iran - Pasteur Institute of Iran - Tehran, Iran , Jahanian-Najafabadi, Ali Department of Pharmaceutical Biotechnology - School of Pharmacy - Isfahan University of Medical Sciences and Health Services - Isfahan, Iran
Abstract :
Baculovirus expression system is one of the most attractive and powerful eukaryotic expression
systems for the production of recombinant proteins. The presence of a biomarker is required to monitor
transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct
a baculovirus expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the
resultant vector was constructed and used for transfection of Spodoptera frugiperda cells. Results: Expression of
the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.
Conclusion: The application of copGFP control bacmid can be considered as an appropriate control for insect cell
transfection.
