Author/Authors :
Razavi Nikoo, h. Laboratory Science Research Center - Golestan University of Medical Sciences - Gorgan, Iran , ardebili, a. Laboratory Science Research Center - Golestan University of Medical Sciences - Gorgan, Iran , ravanshad , m. Department of Virology - Faculty of Medical Sciences - Tarbiat Modares University - Tehran, Iran , rezaei, f. Department of Virology - Faculty of Medical Sciences - Tarbiat Modares University - Tehran, Iran , teimoori , a. Health Research Institute - Infectious and Tropical Diseases Research Center - Ahvaz Jundishapur University of Medical Sciences - Ahvaz, Iran , ajorloo, m. Hepatitis Research Center - Lorestan University of Medical Sciences - Khorramabad, Iran , khanizadeh, s. Hepatitis Research Center - Lorestan University of Medical Sciences - Khorramabad, Iran , pouriayevali, m.h. Department of Hepatitis and AIDS - Pasteur Institute of Iran - Tehran, Iran
Abstract :
Detection and quantification of human Papillomavirus (HPV) genome in oral carcinoma play an
important role in diagnosis, as well as implications for progression of disease. Methods: We evaluated tissues
from 50 esopharyngeal cancers collected from different regions of Iran for HPV E6 using the two type-specific
primers sets. E6 gene of HPV genotypes was amplified by specific primers. The sensitivity of PCR assay was
analyzed and determined using HPV-DNA-containing plasmids. Real-time PCR was utilized to determine the
prevalence and HPV viral load in patients with oral cavity squamous cell carcinoma. Results: Eighteen (36%)
specimens were positive for HPV. Among the 18 positive specimens, 10 showed HPV-18 (55.55%), and 8
specimens were positive for HPV-11 (44.44%). Of the 18 infected specimens, 6 (33.32%) and 12 (66.65%) were
identified as high-titer and low-titer viral load, respectively. Conclusions: The PCR-based assay, developed in the
current study, could be used for HPV detection, quantification, and genotyping in epidemiological and clinical
studies.
