Author/Authors :
Mohamadnia , Abdolreza Virology Research Center - National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Hashemian , Mohammadreza Chronic Respiratory Diseases Research Center - National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Jamaati , Hamidreza Chronic Respiratory Diseases Research Center - National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Bahrami, Naghmeh Craniomaxillofacial Research center - Tehran University of Medical Sciences - Tehran, Iran , Daustany, Mahya Department of Biotechnology - Faculty of Sciences - Islamic Azad University - Tehran, Iran , Tabarsi, Payam Clinical Tuberculosis and Epidemiology Research Center - National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Farzanegan, Behrooz National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Shahid Beheshti University of Medical Sciences - Tehran, Iran
Abstract :
Infectious diseases such as ventilator- associated pneumonia (VAP) are one of the serious
problems in intensive care units (ICU) of hospitals. To date, there has been no appropriate clinical and
diagnostic marker for early detection of this disease. In this study, expression of PIK3R3 and ATp2A1 genes in
patients with VAP were assessed to be used as biomarkers to identify and confirm the disease.
Methods: This study was conducted by using peripheral blood samples of 60 individuals, including 30 patients
with VAP and 30 healthy volunteers. First, the peripheral blood samples were taken and then RNA was
extracted and converted into cDNA. Finally, the assessment of genes was performed by Real-time PCR.
Results: In peripheral blood samples, 46.6% and 30% were positive for PIK3R3 expression in patients and
healthy groups, respectively. The ATp2A1 expression in patients and healthy controls were found 40% and
23.3%, respectively. Comparing the ΔCT obtained for the PIK3R3 and ATp2A1 genes showed statistically
significant differences between the two groups of patients and healthy subjects (p=0.042, p=0.036).
Conclusions: ATp2A1 and PIK3R3 may be used as biomarkers for early detection of VAP disease. However,
further studies are required.
