Lymphocyte Subpopulations in Patients With Acute Brucellosis

The aim of this work was to evaluate changes in lymphocyte subpopulations, especially helper and cytotoxic T cells, inacute brucellosis patients undergoing treatment. Forty-three acute brucellosis patients were included in the study. Twenty healthysubjects served as controls. Total lymphocytes and the CD3+, CD4+, CD8+, CD19+ and CD (16+56)+ subpopulations were counted bytwo-color flow cytometric analysis. The CD4+ counts in patients before and after treatment were not statistically different (p = 0.7),but healthy subjects had significantly more of these cells (p = 0.001 and p = 0.001 compared to pre- and post-treatment patients,respectively). The CD8+ counts in acute brucellosis patients decreased after treatment (p = 0.004), but remained higher in both preandpost-treatment samples than in healthy subjects (p = 0.001 and p = 0.01 respectively). Neither the total leukocyte counts northe numbers of cells in any subpopulation correlated with blood culture results (positive or negative). No statistically significantdifferences in the patientsÕ CD4+ T cell counts were observed between the pre-and post-treatment periods, and the count was higherin healthy subjects. Counts of CD8+ T cells increased in acute brucellosis patients, and although they decreased after treatment theyremained higher than in the controls. In view of this increase, it was concluded that CD8+ T cells could be the major component inimmunity against brucellosis