The effect of microorganisms on cytokine secretion from bone marrow dendritic cells

Aim: Dendritic cells (DCs) are potent antigen-presenting cells crucial for initiation of cell-mediated immune responses to foreign antigens. They also contribute to the innate resistance against microbial pathogens. The present study examined the effect of microorganisms such as Escherichia coli (E. coli), Serratia marcescens (S. marcescens), Methicillin-resistant Staphylococcus aureus (MRSA), coagulase negative staphylococci (CoNS), and Candida albicans (C. albicans) on cytokinesecretion in bone marrow-dendritic cells (BM-DCs).Materials and methods: The levels of cytokines in culture supernatants of the BM-DC were determined by enzyme-linked immunosorbent assay. Results: DCs stimulated by microorganisms produced large amounts of TNF-a, IL-1ß, IL-6, and IL-10. The levels of IFN-?, IL-2, and IL-4 did not increase in the samples with DCs stimulated by microorganisms compared to those of the samples including only DCs. The levels of TNF-a increased remarkably in the DCs stimulated with C. albicans.Conclusion: Our results indicated that gram-negative bacteria tended to induce higher levels of cytokines from DCs than the gram-positive bacteria induced and gram-negative bacteria caused a predominantly inflammatory cytokine response. These results may contribute to the understanding of the role of DC in the mechanisms to prevent infections.