Author/Authors :
KIPROP, E. K. Moi University - Faculty of Science - Department of Botany, Kenya , MWANG OMBE, A. W. University of Nairobi - Faculty of Agriculture - Department of Crop Protection, Kenya , BAUDOIN, J.P. Faculté universitaire des Sciences agronomiques de Gembloux - Phytotechnic tropicale et Horticultures, Belgium , KIMANI, P.M. University of Nairobi - Faculty of Agriculture - Department of Crop Science, Kenya , MERGEAI, G. Faculté universitaire des Sciences agronomiques de Gembloux - Phytotechnic tropicale et Horticultures, Belgium
Abstract :
Thirty-eight isolates of Fusarium udum obtained from pigeonpea (Cajanus cajan) plants showing wilt symptoms were collected from various districts in Kenya and tested for variability in vegetative compatibility groups (VCG) and amplified fragment length polymorphism (AFLP). Nitrate non-utilising mutants were generated from F. udum isolates by selecting chlorate-rcsistant sectors on minimal medium amended with 15 g 11 potassium chlorate. All the isolates of F. udum were grouped into a single VCG (VCG 1) with two subgroups VCG 1I and VCG. 1 II. The DNA of the fungal isolates was extracted using CTAB method. The AFLP analysis of 38 isolates using seven primer combinations generated a total of 318 fragments with 102 being polymorphic (32% polymorphism). The isolates could be grouped into one AFLP group with more than ten subgroups based on the analysis of the banding patterns, although most of these subgroups were not significantly distant ( 50% confidence interval) genetically. Based on VCG and AFLP, the isolates could have originated from a single lineage. The VCG and AFLP of F. udum were independent of geographical origin of the isolates.
