Author/Authors :
TAKUNDWA, M. University of Namibia - Department of Biological Sciences, Namibia , NEPOLO, E. University of Namibia - Department of Biological Sciences, Namibia , MOGOTSI, KHOLA Botswana College of Agriculture - Department of Plant Production, Botswana , KANDAWA-SCHULZ, M.A. University of Namibia - Department of Chemistry and BioChemistry, Namibia , CULLIS, A.C. Case Western Reserve University - Department of Biology, USA , KUNERT, K. University of Pretoria - Department of Plant Science, South Africa , JACKSON-MALETE, J.J. University of Botswana - Centre for Scientific Research, Indigenous Knowledge and Innovation (CESRIKI), Botswana , CHIWONA-KARLTUN, L. Swedish University of Agricultural Sciences - Department of Rural and Urban Development, Sweden , CHIMWAMUROMBE, P.M. University of Namibia - Department of Biological Sciences, Namibia
Abstract :
Marama bean [Tylosema esculentum (Burchell) Schreiber] occurs naturally in arid parts of southern Africa. Due to the high nutrient value of the seeds and tubers; richness in protein, oil and starch; it is a potential crop for arid areas where few conventional crops can survive. Microsatellites are becoming the molecular marker system of choice because they are multiallelic and generally more informative. Recently, the development of SSR enrichment techniques has increased the efficiency of SSR characterisation in new species. The aim of the study was to develop SSR’s for detection of polymorphisms in Marama bean. The microsatellite regions of the genome were the main focus for potential to be used in Marama bean genetic diversity studies. Microsatellite loci were isolated from the Marama bean germplasm using a modified FIASCO enrichment technique. Nine Marama beanmicrosatellite libraries, enriched for (AAG)7, (GTT)7, (AGG)7, (GAG)7, (CA)10, (CT)10, (TCC)7, (CA)15 and (CAC)7, were created. Of the 80 primers designed, 76% were able to detect polymorphism. Four of the SSR’s were used for a genetic variation analysis and have proved to be useful and informative for genetic diversity studies.
Keywords :
FIASCO , polymorphism , SSR , Tylosema esculentum
