A novel glucose chemiluminescence biosensor based on a rhodanine derivative chemiluminescence system and multilayer-enzyme membrane

Using glucose oxidase as a model enzyme, a novel rhodanine derivative chemiluminescence biosensor for the determination of glucose was formed based on multilayer-enzyme membrane as receptor, which was assembled via layer-by-layer assembly of sol-gel and glucose oxidase-gold nano-particles inside a glass tube. Compared with the traditional chemiluminescence biosensor, the proposed biosensor had some remarkable advantages, such as good selectivity of substrate, good response performance, good stability, good sensitivity, and longer service life. The linear range was from 1.0 × 10^−6 to 5.0 × 10^−2 mol L^−1 (r = 0.9991). The detection limit was 5.0 × 10 −7 mol L^−1 . In this work, the optimum number of layers was 4, and the Michaelis-Menten constant of immobilized glucose oxidase was 0.2 mmol L^−1 . The proposed method was applied to the determination of glucose in 6 different human serum samples with satisfactory results.