Determination of Antimicrobial and Antioxidant Activities of Turkish Endemic Salvia halophila Hedge

In this study, the antioxidant activity of ethanol extract of Salvia halophila Hedge (Lamiaceae) was evaluated by 2 antioxidantassays, including phosphomolybdenum reduction and free radical scavenging activity. The ethanol extract showed free radical scavengingactivity with IC50 of 67.73 ìg/mL in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant activity of the extract was found tobe 84.87 ± 0.7 mg ascorbic acid equivalent (AAE)/g extract in phosphomolybdenum assay. In the addition to the antioxidant activityof the extract, the total phenolic content was measured. Total phenolic content of ethanol extract was found to be 8.21 ± 0.0 mg gallicacid equivalent (GAE)/g extract. Antimicrobial activity of the extract was examined against 13 bacteria and 2 yeasts by agar diffusion method. The ethanol extract of S. halophila had no effect against the tested yeasts. The ethanol extract showed antibacterial activityagainst all of the tested Gram (+) bacteria except Listeria monocytogenes at 1%, 2.5%, 5%, and 10% concentrations. The ethanolextract had no effect on the entire tested Gram (-) bacteria except Aeromonas hydrophila and Pseudomonas aeruginosa