Title of article :
Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells
Author/Authors :
YOON, Weon-Jong Jeju High-Tech Development Institute - Jeju Biodiversity Research Institute (JBRI) - Research Group for Cosmetic Materials, KOREA , HAM, Young Min Jeju Sea weed Industry Development Agency, KOREA , HAM, Young Min Jeju High-Tech Development Institute (HiDI) - Jeju Biodiversity Research Institute (JBRI) - Research Group for Cosmetic Materials, KOREA , LEE, Wook Jae Jeju High-Tech Development Institute (HiDI) - Jeju Biodiversity Research Institute (JBRI) - Research Group for Cosmetic Materials, KOREA , LEE, Wook Jae Jeju Seaweed Industry Development Agency, KOREA , LEE, Nam Ho Jeju Seaweed Industry Development Agency, KOREA , LEE, Nam Ho Cheju National University - Department of Chemistry, Korea , HYUN, Chang-Gu Jeju High-Tech Development Institute (HiDI) - Jeju Biodiversity Research Institute (JBRI) - Research Group for Cosmetic Materials, KOREA , HYUN, Chang-Gu Jeju Seaweed Industry Development Agency, KOREA
Abstract :
The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. Theextract was then successively partitioned with n-hexane, CH2Cl2, EtOAc, BuOH, and water. The results indicate that theCH2Cl2 fraction of S. muticumextract was an effective inhibitor of LPS-induced NO and PGE2 production in RAW 264.7cells. These inhibitory effects of the CH2Cl2 fraction of S. muticum included dose-dependent decreases in the expressionof iNOS and COX-2 proteins and iNOS and COX-2 mRNA. To test the inhibitory effects of S. muticum fractions onother cytokines, we also measured IL-1ß and IL-6 mRNA expression by RT-PCR in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the CH2Cl2 fraction of S. muticum decreased the expression of IL-1ß and IL-6 m RNAin a dose-dependent manner. Based on these results, we suggest that S. muticum extracts may be considered possible antiinflammatory candidates for human health.
Keywords :
Algae , extract , inflammation , Sargassum muticum
