Author/Authors :
ZHANG, Li Sichuan Agricultural University - College of Life and Basic Sciences, CHINA , ZHANG, Li Chengdu University of Traditional Chinese Medicine - College of Pharmacy, CHINA , ZHAO, Hong-xia Sichuan Agricultural University - Triticeae Research Institute, CHINA , FAN, Xing Sichuan Agricultural University - Triticeae Research Institute, CHINA , WANG, Meng Sichuan Agricultural University - College of Life and Basic Sciences, CHINA , DING, Chun-bang Sichuan Agricultural University - College of Life and Basic Sciences, CHINA , YANG, Rui-wu Sichuan Agricultural University - College of Life and Basic Sciences, CHINA , YIN, Zhong-qiong Sichuan Agricultural University - College of Life and Basic Sciences, CHINA , XIE, Xian-li Agricultural Bureau of Zhongjiang County, CHINA , ZHOU, Yong-hong Sichuan Agricultural University - Triticeae Research Institute, CHINA , WAN, De-guang Chengdu University of Traditional Chinese Medicine - College of Pharmacy, CHINA
Abstract :
To investigate the genetic diversity and phylogenetic relationships of Salvia miltiorrhiza and related species, we analyzed the nuclear ribosomal DNA internal transcribed spacer (ITS) region for 7 accessions of Salvia miltiorrhiza and another 23 samples from other taxa within the genus Salvia by maximum parsimony and Bayesian inference analyses. There were 257 variation sites amounting to 40.8% of the total base pairs. All of the data revealed abundant genetic diversity in the genus Salvia. The results showed that the tested materials could be grouped into 3 clusters, and the 3 different occasions in Salvia were clustered into 1 clade, which suggests that the species from different occasions form independent lineages. We can easily distinguish S. miltiorrhiza from a variety of Salvia species in this manner. Moreover, according to cluster analysis, S. bowleyana, S. yunnanensis, and S. cavaleriei var. simplelefolia are good or potential germplasm resources for S. miltiorrhiza.
