Title of article
A comparative study of EGF effects on in vitro bovine embryo development in monoculture and sequential media
Author/Authors
ZAHMATKESH, Azadeh isfahan university of technology - College of Agriculture - Department of Animal Sciences, اصفهان, ايران , AMIRI ROUDBAR, Mahmood ferdowsi university of mashhad - College of Agriculture - Department of Animal Sciences, مشهد, ايران , DALIRI JOUPARI, Morteza National Institute of Genetic Engineering and Biotechnology - Department of Animal and Marine Biotechnology, ايران
From page
670
To page
674
Abstract
Different culture system conditions can affect cleavage, zygote genome activation, embryo development, and viability. The purpose of the present study was to compare a monoculture synthetic oviductal fluid medium with modified sequential G1/G2 media and also to investigate the effect of epidermal growth factor (EGF) on bovine embryo development. Oocytes were aspirated from 2- to 6-mm ovarian follicles and matured in maturation media. After in vitro fertilization of matured oocytes, presumptive zygotes were cultured in test media with or without additional exogenous EGF. Results indicated that EGF significantly increased blastocyst formation but not cleavage rate in both media. There was no significant difference between the sequential and the monoculture media.
Keywords
Blastocyst , cleavage , culture system , epidermal growth factor
Journal title
Turkish Journal of Biology
Journal title
Turkish Journal of Biology
Record number
2534196
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