Author/Authors :
MOZİOĞLU, Erkan TÜBİTAK National Metrology Institute (UME) - Bioanalysis Laboratory, Turkey , MOZİOĞLU, Erkan Istanbul Technical University - Molecular Biology, Biotechnology, and Genetics Research Center, Turkey , AKGÖZ, Müslüm TÜBİTAK National Metrology Institute (UME) - Bioanalysis Laboratory, Turkey , TAMERLER, Candan University of Kansas - Mechanical Engineering Department, USA , TAMERLER, Candan Istanbul Technical University - Molecular Biology, Biotechnology, and Genetics Research Center, Turkey , KOCAGÖZ, Zühtü Tanıl Acıbadem University - Department of Microbiology and Clinical Microbiology, Turkey , KOCAGÖZ, Zühtü Tanıl Trends in Innovative Biotechnology Organization, Turkey
Abstract :
A high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study,we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp.enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. The results show that the modified guanidinium isothiocyanate method developed in our laboratory is simple, fast, and inexpensive and yields DNA whose quality and quantity are similar to that of 2 commercial extraction kits but far better than 2 conventional DNA extraction methods used for comparison.
