Author/Authors :
UZUN, Satı Erciyes University - Seyrani Agricultural Faculty - Department of Field Crops, Turkey , PARMAKSIZ, İskender Gaziosmanpaşa University - Faculty of Science and Arts - Department of Molecular Biology and Genetics, Turkey , URANBEY, Serkan Çankırı Karatekin University - Faculty of Science - Department of Biology, Turkey , MİRİCİ, Semra Gazi Üniversitesi - Gazi Education Faculty - Department of Biology Education, Turkey , SARIHAN, Ercüment Osman Uşak University - Faculty of Agriculture and Natural Sciences - Department of Field Crops, Turkey , İPEK, Arif Çankırı Karatekin University - Faculty of Science - Department of Biology, Turkey , KAYA, Mehmet Demir Eskişehir Osmangazi University - Faculty of Agriculture - Department of Field Crops, Turkey , GÜRBÜZ, Bilal Ankara Üniversitesi - Faculty of Agriculture - Department of Field Crops, Turkey , ARSLAN, Neşet Ankara Üniversitesi - Faculty of Agriculture - Department of Field Crops, Turkey , SANCAK, Cengiz Ankara Üniversitesi - Faculty of Agriculture - Department of Field Crops, Turkey , KHAWAR, Khalid Mahmood Ankara Üniversitesi - Faculty of Agriculture - Department of Field Crops, Turkey , ÖZCAN, Sebahattin Ankara Üniversitesi - Faculty of Agriculture - Department of Field Crops, Turkey
Abstract :
An efficient in vitro bulblet production procedure from immature zygotic embryos of endemic and endangered Muscari muscarimi Medik. was described in the current study. Zygotic embryos were first isolated from immature seeds and cultured on different nutrient media compositions supplemented with various combinations of α-naphthalene acetic acid (NAA), picloram, dicamba, 6-benzylaminopurine (BAP), and thidiazuron (TDZ). The best bulblet regeneration (59 bulblets per explant) was achieved in Murashige and Skoog (MS) medium containing 4 mg/L BAP and 0.5 mg/L NAA after 1 year of culture initiation. Regenerated bulblets were then transferred into MS medium without plant growth regulators for rooting. Bulblets produced well-developed root systems and increased their size on this medium after 2 months. All rooted bulblets were successfully transplanted into a potting mixture and acclimatized to ambient conditions.
