Squalestatin-induced production of taxol and baccatin in cell suspension culture of yew (Taxus baccata L.)

Various elicitors have already been reported to enhance the production of taxol and related taxanes. In this study, effects of a fungal metabolite, squalestatin, on production of taxol and baccatin III were studied. Expressions of 4 key involved genes, ts, dbat, bapt, and dbtnbt, in suspension cultures of Taxus baccata were also detected using qPCR. Results showed that application of squalestatin significantly increased taxol and baccatin III yields. Increased expressions of the genes were in accordance with measures of taxol and baccatin accumulations in cells and medium. Production of H2O2 has significant positive correlations with both gene expression and taxanes, indicating that the increase in H2O2 might be involved in the upregulation of the taxane production in yew under squalestatin treatment. Our results suggest that H2O2 is a key signaling component in the stimulation of taxane production in T. baccata cells induced by squalestatin.