Author/Authors :
LAN, Tao Fujian Agriculture and Forestry University - College of Crop Science, CHINA , LAN, Tao Key Laboratory of Ministry of Education for Genetics, Breeding, and Multiple Utilization of Crops, China , LAN, Tao Fujian Provincial Key Laboratory of Rice Molecular Marker-Assisted Breeding, CHINA , DUAN, Yuanlin Key Laboratory of Ministry of Education for Genetics, Breeding, and Multiple Utilization of Crops, CHINA , DUAN, Yuanlin Fujian Agriculture and Forestry University - College of Crop Science, CHINA , DUAN, Yuanlin Fujian Provincial Key Laboratory of Rice Molecular Marker-Assisted Breeding, CHINA , WANG, Bin Fujian Agriculture and Forestry University - College of Life Science, CHINA , WANG, Bin Key Laboratory of Ministry of Education for Genetics, Breeding, and Multiple Utilization of Crops, CHINA , ZHOU, Yuanchang Key Laboratory of Ministry of Education for Genetics, Breeding, and Multiple Utilization of Crops, CHINA , ZHOU, Yuanchang Fujian Provincial Key Laboratory of Rice Molecular Marker-Assisted Breeding, CHINA , ZHOU, Yuanchang Fujian Agriculture and Forestry University - College of Crop Science, CHINA , WU, Weiren Key Laboratory of Ministry of Education for Genetics, Breeding, and Multiple Utilization of Crops, CHINA , WU, Weiren Fujian Provincial Key Laboratory of Rice Molecular Marker-Assisted Breeding, CHINA , WU, Weiren Fujian Agriculture and Forestry University - College of Life Science, CHINA
Abstract :
Na^+/H^+ antiporters have been suggested to play important roles in salt tolerance in plants. English cordgrass (Spartina anglica) is a gramineous halophyte with very strong salt tolerance. It possesses salt glands in its stems and leaves, through which excess salt can be excreted. In this study, a vacuolar Na^+/H^+ antiporter homologous gene (temporarily named SaNHX1) was isolated from Spartina anglica by RT-PCR and rapid amplification of cDNA ends (RACE). Southern blot analysis suggested that there might be 2 or 3 copies of the vacuolar Na^+/H^+ antiporter genes in the English cordgrass genome. Northern blot analysis showed that the expression of vacuolar Na^+/H^+ antiporter genes in English cordgrass is induced by salt stress. Overexpression of SaNHX1 driven by constitutive promoter Ubi-1 in rice significantly enhanced the salt tolerance of transgenic plants, validating the function of SaNHX1 and suggesting its value for the genetic improvement of salt tolerance in plants.
