Author/Authors :
Lucas, Esther M. F. Universidade Federal de Minas Gerais - Instituto de Ciências Exatas (ICEx) - Departamento de Química, Brazil , Lucas, Esther M. F. Universidade Federal de Lavras, Campus Universitário - Departamento de Química, Brazil , Machado, Yuri Universidade Federal de Minas Gerais - Instituto de Ciências Exatas (ICEx) - Departamento de Química, Brazil , Ferreira, Alexandre A. Universidade Federal de Minas Gerais - Instituto de Ciências Exatas (ICEx) - Departamento de Química, Brazil , Dolabella, Luiza M. P. Universidade Federal de Minas Gerais - Instituto de Ciências Exatas (ICEx) - Departamento de Química, Brazil , Takahashi, Jacqueline A. Universidade Federal de Minas Gerais - Instituto de Ciências Exatas (ICEx) - Departamento de Química, Brazil
Abstract :
Purpose: The aim of this work was to study the optimum conditions for the production of sclerotiorin, a highly active secondary metabolite of Penicillium sclerotiorum under different cultural media. Methods: A Brazilian strain of P. sclerotiorum was grown under different culture conditions in two liquid media (malt and a dextrose-peptone salt medium supplemented with sodium chloride) and in solid state fermentation in rice. Sclerotiorin production was monitored by high performance liquid chromatography (HPLC). Results: Quantitative analysis of sclerotiorin content by HPLC indicated that sclerotiorin production reached the highest level (up to 313 + 10 mg.L^-1) in the dextrose-based medium after 10 days of fermentation. Rice and malt broth showed lower production levels. Conclusion: Enhanced production of P. sclerotiorin for pharmaceutical development can be achieved using dextrose-based cultures.
Keywords :
Penicillium sclerotiorum , Sclerotiorin , Yield improvement , HPLC , Pharmaceutical industry
