Title of article :
Cisplatin Induces Up-Regulation of KAI1, a Metastasis Suppressor Gene, in MCF-7 Breast Cancer Cell Line
Author/Authors :
Mokhtari, Mohammad Javad islamic azad university - Department of Biology, ايران , Akbarzadeh, Azim Pasteur Institute of Iran - Biotechnology Research Center - Pilot Biotechnology Department, ايران , Hashemi, Mehrdad islamic azad university - Department of Biology, ايران , Javadi, Gholamreza islamic azad university - Department of Biology, ايران , Mahdian, Reza Pasteur Institute of Iran - Biotechnology Research Center - Department of Molecular Medicine, ايران , Ghasemi, Soheil Pasteur Institute of Iran - Biotechnology Research Center - Pilot Biotechnology Department, ايران , Kamiab, Ahmad Reza islamic azad university - Department of Biology, ايران , Hoseineian, Zhilla islamic azad university - Department of Biology, ايران , Chiani, Mohsen Pasteur Institute of Iran - Biotechnology Research Center - Pilot Biotechnology Department, ايران , Taghavi, Mahdieh Sadat islamic azad university - Department of Biology, ايران
Abstract :
Purpose: To investigate the effect of cisplatin on cell toxicity and metastasis through modulation of KAI1 gene expression. Methods: MCF-7cells were incubated with different concentrations of cisplatin for 24 h. RNA was extracted by trizol and cDNA synthesized. KAI1 and TBP were chosen as target and internal control genes, respectively. Specific primers were designed by primer express software, v.3.0. KAI1/TBP and gene expression ratio was calculated using the formula, 2^ -ΔΔCt.Results: Cisplatin exerted a dose-dependent inhibitory effect on the viability of highly metastatic MCF-7 cells. KAI1/TBP gene expression ratios were 1.97 ± 0.19 (p 0.05), 2.96 ± 0.55 (p 0.05), 9.06 ± 0.27 (p 0.001) and 12.38 ± 0.88 (p 0.01) in 10, 20, 50 and 100 μM concentrations of cisplatin. Conclusion: These findings indicate that cisplatin can inhibit metastasis by up-regulating KAI1 gene in MCF-7cells.
Keywords :
Cisplatin , KAI1 , Metastasis , Breast Cancer , Real , time PCR.
