Author/Authors :
Kim, Moo-Sung Macrocare Tech, Ltd - R D Center, south korea , Koppula, Sushruta Konkuk University - College of Biomedical and Health Science, Research Institute for Biomedical Health Science - Department of Biotechnology, south korea , Jung, Seung-Hyo Konkuk University - College of Biomedical and Health Science, Research Institute for Biomedical Health Science - Department of Biotechnology, South Korea , Jung, Seung-Hyo Macrocare Tech, Ltd - R D Center, South Korea , Kim, Ji-Young Konkuk University - College of Biomedical and Health Science, Research Institute for Biomedical Health Science - Department of Biotechnology, South Korea , Kim, Ji-Young Macrocare Tech, Ltd - R D Center, South Korea , Lee, Hyoung-Ro Konkuk University - College of Biomedical and Health Science, Research Institute for Biomedical Health Science - Department of Biotechnology, South Korea , Lee, Hyoung-Ro KuGen Healthcare Institute, South Korea , Lee, Sang-Rin Macrocare Tech, Ltd - R D Center, south korea , Park, Yong-Dae Macrocare Tech, Ltd - R D Center, south korea , Lee, Kyung-Ae Macrocare Tech, Ltd - R D Center, south korea , Park, Tae-Kyu Konkuk University - College of Biomedical and Health Science, Research Institute for Biomedical Health Science - Department of Biotechnology, south korea , Kang, Hyun Konkuk University - College of Biomedical and Health Science, Research Institute for Biomedical Health Science - Department of Biotechnology, south korea
Abstract :
Purpose: To investigate the antioxidant and anti-neuroinflammatory potentials of Olea europaea Linn. fruit pulp (OFP-EA) extract in LPS-stimulated BV-2 microglial cells. Methods: Cell viabilities were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl-tetrazolium bromide (MTT) assay. Antioxidant properties were evaluated using 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity. Lipopolysaccharide (LPS) was used to stimulate BV-2 microglia. Nitric oxide (NO) production was measured using Griess assay. Inducible NO synthase (iNOS) expression and tumor necrosis factor-alpha (TNF-α) production were measured using enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Results: OFP-EA extract significantly (p 0.001 at 20-200 μg/ml, respectively) scavenged the free radicals in a dose-dependent fashion. The increased levels of No stimulated by LPS (34±2.41) were also inhibited by OFP-EA extract significantly and concentration dependently (27±2.32, 21±2.54, 17±1.92 and 11±1.94 at 10, 20, 40 and 80 μg/ml, respectively). Further, OFP-EA suppressed the elevated levels iNOS expression and TNF-α production (p 0.001 at 20, 40 and 80 μg/ml) in LPSstimulated BV-2 cells. Conclusion: Results indicate that OFP-EA extract exhibited strong antioxidant properties and inhibited the excessive production of pro-inflammatory mediators such as NO, iNOS and TNF-α in LPSstimulated BV-2 cells. The antioxidant activity exhibited by OFP-EA extract might play a critical role in ameliorating the inflammatory processes in LPS-stimulated BV-2 microglial cells.
Keywords :
Olive fruit pulp , antioxidant , neuroinflammation , microglia , TNF , α , iNOS.
