Gas Chromatography-Mass Spectrometric Analysis of Essential Oil of Aerial Parts of Glycosmis parviflora (Sims) Little (Rutaceae)

Purpose: To investigate the chemical composition and toxicity of the essential oil of Glycosmis parviflora (Sims) Little aerial parts against root-knot nematode and two grain storage insects (maizeweevil and red flour beetle). Methods: Steam distillation of G. parviflora was carried out using Clavenger apparatus in order to extract its volatile oil. Gas chromatography/mass spectrometric (GC/MS) analysis (HP-5MS column) of the essential oil was performed and the toxicity of the oil determined by contact test. Results: A total of 37 components comprising 98.7 % of the essential oil were identified, of which (Z)-caryophyllene (20.6 %), methyl isoeugenol (11.1 %), (Z)-β-ocimene (8.9 %), α-cubebene (6.4 %), nerolidol (5.4 %), aromandendrene (4.9 %) and γ-pyronene (4.7 %) were found to be the major components. The essential oil possessed strong nematicidal activity against M. incognita with an LC50 value of 92.84 μg/ml. The essential oil of G. parviflora exhibited strong contact toxicity against S. zeamais and T. castaneum adults with LD50 values of 41.7 and 22.6 μg/adult, respectively. Conclusion: The study indicates that the essential oil of G. parviflora aerial parts has a potential for development into a natural insecticide/nematicide for control of nematodes and grain storage insects.