Author/Authors :
ZAROUK, WAHEBA A. National Research Centre - Department of Molecular Genetic Enzymology, Egypt , HUSSEIN, IBTESSAM RAMZY National Research Centre - Department of Molecular Genetic Enzymology, Egypt , ABDEL REHIEM, HANAN A. Cairo University - Faculty of Medicine - Department of Internal Medicine, Egypt , RASLAN, HALA M. National Research Centre - Department of Internal Medicine, Egypt , EMARA, NAHED A. National Research Center - Department of Clinical Chemical Pathology, Egypt , RASHEED, MAHA A. National Research Centre - Department of Clinical Chemical Pathology, Egypt , EL WASSEFE, MAHA National Research Centre - Department of Medical Biochemistry, Egypt
Abstract :
Objective: The deletion (D) allele of the angiotensin-I converting enzyme (ACE) is associated with higher ACE activity, it has been studied in various populations in relation to hypertension and type 2 diabetes mellitus (DM) with contradictory results. The objective of this study was to determine the ACE insertion/deletion polymorphism , genotype distribution in Egyptian patients with type 2 DM and to evaluate the possible association of ACE insertion/deletion polymorphism with hypertension in diabetic patients. Subjects and Methods: A total of 48 patients with type 2 DM, 23 of them had hypertension and 21 healthy subjects age and sex matched with the patients, as control group were included in this study. Genotyping was performed by polymerase chain reaction (PCR). Results: The frequency of DD genotype was significantly higher in diabetic patients compared to controls (p=0.008). The DD genotype (Vs DI and II genotypes) was associated with increased risk of diabetes (OR: 3.647, 95% CI: 1.235- 10.773, p=0.016) and the D allele was more frequent in diabetic patients and was associated with increased risk of diabetes (OR: 3.939, 95% CI: 1.782-8.709, p 0.001). No significant difference in genotype distribution or allele frequency was detected between diabetic patients with and without hypertension. Conclusion: We can conclude that a significant association between ACE gene I/D polymorphism and type 2 DM is present in Egyptian patients and the D allele is associated with increased risk for type 2 DM.
