Author/Authors :
KOTB, MAHA M. Cairo University - Faculty of Medicine - Department of Medical Microbiology Immunology, Egypt , SEDKY, NAGWA A. Cairo University - Faculty of Medicine - Department of Medical Microbiology Immunology, Egypt , EL-SEIDI, EMAN A. Cairo University - Faculty of Medicine - Department of Medical Microbiology Immunology, Egypt , AWAD, ALAA R. Cairo University - Faculty of Medicine - Department of Medical Microbiology Immunology, Egypt , AL-WAKEEL, SAHAR S. Cairo University - Faculty of Medicine - Department of Internal Medicine, Egypt
Abstract :
Aim: This study aimed to determine the presence of carbapenem resistance among Enterobacteriaceae by disk diffusion method and Epsilometer test (E-test).Methodology: In the present study, 100 Enterobacteriaceae were isolated from different clinical samples and identified by Analytical Profile Index (API) 20 E. Antimicrobial susceptibility testing was done by modified kirby bauer disk diffusion method; using carbapenems; Ertapenem (ETP) and Meropenem (MEM) and third generation cephalosporins; Cefoprazone (CFP) and Cefotaxime (CTX) and followed by Minimum Inhibitory Concentration (MIC) determination by E-test for ETP and MEM.Results: The disk diffusion method showed that 8 1 % of the isolates were resistant to CTX, 79% were non-susceptible (i.e. resistant and intermediate isolates) to CFP, and the carbapenem non-susceptibility was 68 isolates (68%) to ETP and 57 isolates (57%) to MEM. MIC determination results by E-test method differed from disk diffusion results and showed that the carbapenem non-susceptibility to ETP and MEM was 19% and 9% respectively.Conclusions: The presence of carbapenem resistance among Enterobacteriaceae isolates causing healthcare-associated infections in the current study emphasizes the necessity for early detection of these isolates and reporting to infection prevention staff to overcome their spread.
