Author/Authors :
KOTB, MAHA M. Cairo University - Faculty of Medicine - Department of Medical Microbiology Immunology, Egypt , SEDKY, NAGWA A. Cairo University - Faculty of Medicine - Department of Medical Microbiology Immunology, Egypt , EL-SEIDI, EMAN A. Cairo University - Faculty of Medicine - Department of Medical Microbiology Immunology, Egypt , AWAD, ALAA R. Cairo University - Faculty of Medicine - Department of Medical Microbiology Immunology, Egypt , AL-WAKEEL, SAHAR S. Cairo University - Faculty of Medicine - Department of Internal Medicine, Egypt
Abstract :
Aim: This study aimed to detect the presence of Klebsiella Pneumoniae Carbapenemase (KPC) enzyme among Enterobacteriaceae by phenotypic tests; the Modified Hodge Test (MHT) and boronic acid inhibition test and to compare both methods to genotypic detection of blaKPC gene by Polymerase Chain Reaction (PCR).Methodology: This study involved detection of KPC among 72 carbapenem non-susceptible isolates of enterobac-teriaceae by phenotypic methods as MHT and boronic acid inhibiton test followed by genotypic methods as PCR.Results: The results of this study showed that the prevalence of carbapenemase producing isolates by MHT was 51% and prevalence of KPC by carbapenem-boronic acid combined disk test was 35%, while it was 49% by genotypic method.Conclusions: The presence of Klebsiella Pneumoniae Carbapenemase among enterobacteriaceae isolates causing healthcare-associated infections in the current study emphasizes the necessity for early detection of these isolates by phenotypic tests and genotypic tests.
