Author/Authors :
Saigo، Katsuyasu نويسنده , , Sugimoto، Takeshi نويسنده , , Takeuchi، Satoshi نويسنده , , Kosaka، Yoshiyuki نويسنده , , Ryo، Ryukichi نويسنده , , Kumagai، Shunichi نويسنده ,
Abstract :
We inquired whether stem cell fractions in peripheral blood stem cell harvest could be precisely detected by using a hematopoietic progenitor cell (HPC) counting system applied to an automated hematology analyzer, SE-9000. Although there was an apparent increase in the HPCs 20 h after storage in nondiluted conditions, samples diluted with RPMI-1640 containing 1.0 mg/ml EDTA-2K showed a relatively stable number of HPCs. There was a significant relationship between HPCs and CD34 cells (n = 75, r = 0.769). This method may represent the least expensive and most time-effective way for stem cell estimation in harvest products.
