Author/Authors :
Lefterova، Petja نويسنده , , M?rten، Angela نويسنده , , Buttgereit، Peter نويسنده , , Schakowski، Frank نويسنده , , Micka، Bettina نويسنده , , Scheffold، Christian نويسنده , , Schmidt-Wolf، Ingo G.H. نويسنده ,
Abstract :
We inquired whether stem cell fractions in peripheral blood stem cell harvest could be precisely detected by using a hematopoietic progenitor cell (HPC) counting system applied to an automated hematology analyzer, SE-9000. Although there was an apparent increase in the HPCs 20 h after storage in nondiluted conditions, samples diluted with RPMI-1640 containing 1.0 mg/ml EDTA-2K showed a relatively stable number of HPCs. There was a significant relationship between HPCs and CD34 cells (n = 75, r = 0.769). This method may represent the least expensive and most time-effective way for stem cell estimation in harvest products.
