Title of article
Production of effective antivenin to treat cobra snake(Naja naja oxiana) envenoming
Author/Authors
Akbari, A. Razi Vaccine and Serum Research Institute - Department of venomous animals and anti venom production, ايران , Rabiei, H. Razi Vaccine and Serum Research Institute - Department of venomous animals and anti venom production,, ايران , Hedayat, A. Razi Vaccine and Serum Research Institute - Department of Venomous animals and Antivenin production, ايران , Mohammadpour, N. Razi Vaccine and Serum Research Institute - Department of venomous animals and anti venom production, ايران , Zolfagharian, H. Razi Vaccine and Serum Research Institute - Department of Antivenin and Venomous animals, ايران , Teimorzadeh, Sh. Razi Vaccine and Serum Research Institute - Department of venomous animals and anti venom production, ايران
From page
33
To page
37
Abstract
Conventional treatment of Naja naja oxiana (NNO) envenoming requires large volumes of equine antivenin raised against NNO crude venom. The poor efficiency of this antivenin is assumed to be due to the high molecular weight non-toxic proteins, a strong immunogen, present in the crude venom. These proteins cause depression of antibody formation against the low molecular weight toxic components of venom. In the present study the low molecular weight lethal components were isolated from crude venom of NNO venom by sephadex G50 get filtration chromatography. A sera was prepared by immunizing horses with toxic fraction. One milliliter of this serum neutralized 1.8mg of NNO crude venom. This high titer antivenin is thus 2.2 times more potent than the sera obtained against NNO crude venom.
Keywords
Naja naja Oxiana , venom , gel chromatography , Antivenin
Journal title
Archives of Razi Institute
Journal title
Archives of Razi Institute
Record number
2545770
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